Figure 2. Complementation of a D.discoideum aca- mutant by mPAC.
Wild-type D.discoideum, aca- and aca-/A15::mPAC-YFP cells were plated on non-nutrient agar at a density of 1.5×106 cells/cm2 and incubated under ambient room lighting or in darkness as indicated. Developmental progression was recorded at 5 h intervals. For wild-type and aca- cells, development or lack thereof was the same in darkness or in light.