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. Author manuscript; available in PMC: 2016 Apr 23.
Published in final edited form as: Cell. 2015 Apr 23;161(3):438–449. doi: 10.1016/j.cell.2015.03.050

Figure 2. Single-Particle Cryo-EM Images with Motion Correction.

Figure 2

Most data recorded with DDD cameras are dose-fractionated image stacks (movies) that can be motion-corrected.

(A) A typical cryo-EM image of vitrified archaeal 20S proteasome particles embedded in a thin layer of vitreous ice. The image is the sum of the raw movie frames without motion correction.

(B) Trace of motion of all movie frames determined using a whole-frame motion-correction algorithm (Li et al., 2013a). Note that the movement between frames is large at the beginning but then slows down.

(C) The left panel shows the power spectrum calculated from the sum of the raw movie frames without motion correction. The right panel shows the power spectrum calculated from the sum of movie frames after motion correction. Motion correction restores Thon rings to close to 3Å resolution (dashed circle).

(D) Sum of the movie frames that were shifted according to the shifts shown in (B). Note that the images shown in (A) and (D) are indistinguishable by eye, but differ significantly in the quality of the Thon rings seen in their power spectra (C).