Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Apr 20;16(1):326. doi: 10.1186/s12864-015-1526-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Lichius et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Gene replacement with the truncated GFP-XYR1^1–780 reproduced the XYR1-loss-of-function phenotype in QM9414. (A) QM9414::GFP-XYR1^1–780 transformants (TRAL007 clones 1 to 3) showed the same growth defect on 65 mM D-xylose as QM9136 and ∆xyr1, respectively, indicating loss of XYR1 function, which was further verified by the corresponding macroscopic colony phenotypes, shown in (B). (C) Gene expression of cellulase cel7a, xylanase xyn2 and xyr1 were not significantly upregulated in GFP-XYR1^1–780 expressing transformants (TRAL007), whereas the GFP-XYR1 transformants (TRAL002) clearly responded to the inductive stimulus provided by sophorose. Error bars show standard deviation (n = 6 from three experimental and two biological replicates involving two different TRAL007 clones).