Table 3.
Oligonucleotides used in this study for PCR genotyping and DNA sequencing
| Primer name | 5′-3′ sequence |
|---|---|
| PCR genotyping | |
| Pxyr1-ver-F | AAGGATGCCGACTTAACGAAC |
| Txyr1-ver-R | AGTCGCTCATGATCCTACCAG |
| Xyr1-ver-R | CCTGGCAGCAATAAGAGAGC |
| Xyr1-ver-F | CCTTGCGGATAAGTGGGATC |
| 5Pxyr1-ver-F | CCAGCTGCCACTCTCATG |
| GFP-ver-R | AAGCACTGCACGCCGTA |
| GFP-ver-F | TACGGCGTGCAGTGCTTC |
| Pgpdh-ver-F | TGCTAAGGTACCTAGGGAGGGA |
| hph-ver-R | CAAGCACTTCCGGAATCG |
| act-mp-F | ACTTTCGGCCGCATTCTG |
| act-mp-R | AGCCAGGATCTTCATCAGGTAG |
| DNA sequencing | |
| Pxyr1-seq-F | GACAGCAGCAGTAGTCAGGT |
| Txyr1-seq-F | TTGCTGAAAGTGAAGAGGG |
| xyr1-seq-F | CCGTCTCCCAAGACTAGC |
| 5′xyr1-seq-F | CACATTGAGGGCTCTGTC |
| 3′xyr1-seq-F | AAGCATCTGCCATTGTCC |
| Xyr1∆A2294-seq-F | GCCACGATGCAGGGAA |
| 3′GFP-seq-F | CCGACAACCACTACCTGA |
| 5′GFP-seq-F | GCCACAAGTTCAGCGTG |
| Pgpdh-seq-F | CATGCAGTTGCTAAGGTACC |
| Tgpdh-seq-R | GACTGTGAGCATGTGATGGC |