Table 3.
Validation of the NDCUH database
| No. of isolates | Before the NDCUH introduction | After the NDCUH introduction | |||||
|---|---|---|---|---|---|---|---|
| Species level | Genus level | No match | Species level | Genus level | No match | ||
| N. abscessus | 2 | 2 | 1 | 1 | |||
| N. aobensis | 2 | 1 | 1 | 2 | |||
| N. arthritidis | 2 | 2 | 1 | 1 | |||
| N.a asiatica | 3 | 2 | 1 | 3 | |||
| N. asteroides | 2 | 2 | 2 | ||||
| N. beijingensis | 2 | 2 | 2 | ||||
| N. brasiliensis | 2 | 2 | 2 | ||||
| N. concava | 2 | 1 | 1 | 1 | 1 | ||
| N. cyriacigeorgica | 4 | 4 | 4 | ||||
| N. elegans | 4 | 3 | 1 | 4 | |||
| N. exalbida | 2 | 2 | 2 | ||||
| N. farcinica | 7 | 7 | 7 | ||||
| N. niigatensis | 2 | 2 | 2 | ||||
| N. nova | 8 | 5 | 3 | 7 | 1 | ||
| N. otitidiscaviarum | 3 | 2 | 1 | 3 | |||
| N. paucivorans | 1 | 1 | 1 | ||||
| N. pseudobrasiliensis | 2 | 2 | 1 | 1 | |||
| N. puris | 3 | 3 | 3 | ||||
| N. transvalensis | 2 | 1 | 1 | 1 | 1 | ||
| N. veterana | 2 | 1 | 1 | 2 | |||
| N. vinacea | 2 | 2 | 2 | ||||
| N. wallacei | 5 | 5 | 5 | ||||
| Total | 64 | 10 (15.6%) | 16 (25.0%) | 38 (59.4%) | 58 (90.6%) | 6 (9.4%) | 0 (0.0%) |
A total of 64 Nocardia isolates, independent of the isolates used to construct the NDCUH, were subjected to identification based on the NECLC method; identification results before and after introduction of the NDCUH database were compared. Before the introduction, the original Bruker Biotyper ver. 3.3.1.0 database was used. In the table, the species level count indicates isolates that could be identified at both the species and genus levels; the genus level count includes not only isolates that could be identified to the genus level but those that could not be identified to the species level.