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Identification of Dll4-AS promoter. (A) Genomic fragments aligning with both Dll4 and Dll4-AS transcription start sites were cloned into a promoter reporter vector, pGL4.14. (B) These constructed vectors were mixed with 1/20 (molar ratio) Renilla vector, which serves as internal control for cell transfection. The transfected MS1 cells were lysed 48 h later for luminescence reading.
