Table 3.
NAI Inhibition of agglutination of MDCK cultured H3N2 viruses and NA 148 and 151 sequences
| Name | CRBC osel a | CRBC zan a | TRBC osel | TRBC zan | Amino acid 151 b | Mutant percentage | Amino acid 148 b | Mutant percentage | Sequence coverage |
|---|---|---|---|---|---|---|---|---|---|
| A/Victoria/4/94 | 1-10 | 10-100 | >1000 | >1000 | - | - | - | ||
| A/Auckland/5/96 | 1-10 | 1-10 | >1000 | >1000 | D | T/I | 94% | 256 | |
| A/Victoria/54/96 | 10 | 10 | 10 | 10 | D/G | 40% | T | 560 | |
| A/Auckland/19/96 | 1-10 | 1-10 | >1000 | >1000 | D | T/I | 14% | 230 | |
| A/Victoria/6/97 | 10 | 10-100 | >1000 | >1000 | D/G | 10% | T | 500 | |
| A/Victoria/314/98 | 1-10 | 1-10 | 10 | 10 | D | T/I | 10% | 250 | |
| A/Victoria/3/99 | 10 | 10-100 | >1000 | >1000 | D/N | 37% | T | 300 | |
| A/Victoria/514/00 | 10 | 10-100 | 10 | 100 | D/N | 17% | T/I | 12% | 388 |
| A/Perth/201/01 | ND | ND | >1000 | >1000 | D/N | 14% | T | 535 | |
| A/Perth/200/02 | 10 | 10 | 10 | 10 | D/N | 6.5% | T/I | 88% | 1283 |
| A/Christchurch/28/03 | 10 | 10 | 10 | 10-100 | D/G | 10% | T/K | 24% | 784 |
| A/Wellington/1/04 | 10 | 10 | 10 | 10 | D | I | 100% | 1005 | |
| A/Brisbane/3/05 | 10 | 10-100 | 10 | 10-100 | D/N/G | 27%/10% | T | 630 | |
| A/Victoria/503/06 | 10 | 10-100 | 10 | 10-100 | D/N/G | 16%/11% | T | 870 | |
| A/Brisbane/10/07 | 10 | 10 | 10 | 10 | D/G | 39% | T | 1193 | |
| A/Brisbane/2/08 | 1-10 | 10-100 | 10 | 10-100 | D/N | 34% | T | 1058 |
aFour HA units of virus were preincubated with log10 dilutions of oseltamivir (osel) or zanamivir (zan) for 60 min at 4°C prior to the addition of 1% RBCs. Titers are the minimum NAI concentration required to completely inhibit agglutination of chicken RBCs in the NAI-HI assay. Where consecutive drug dilutions showed partial then full inhibition a range between these two is given.
bAmino acids at position 151 and 148 based on Ion Torrent PGM deep sequencing.