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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: J Mol Cell Cardiol. 2015 Jan 30;81:81–93. doi: 10.1016/j.yjmcc.2015.01.013

Figure 5. Manipulations of RyR2, and NCX and its efficacy on spontaneous contraction in hiPS-CMs.

Figure 5

A: K201 (2 μM), a selective RyR2 stabilizer gradually decreased the frequency of spontaneous contraction in a cluster of hiPS-CMs and eventually terminated spontaneous contraction (n=5/5). HiPS-CMs remained excitable by electrical stimulation. After 2 min of K201 exposure, a ~2-fold decrease in frequency of automaticity was statistically significant (right panel)

B: Similar effects of 5μM tetracaine infusion on spontaneous contraction in hiPS-CMs

C: SEA0400 (2 μM), an inhibitor of the forward mode of NCX suppressed spontaneous contractions in a cluster of iPS-CMs; an effect that was reversible by washing out SEA0400 (right trace) (n=4/4).