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Mapping the late stages of communesin biosynthetic pathway. Confirming the roles of enzymes encoded by (A) cnsE (methyltransferase), (B) cnsI (PKS), (C) cnsJ (epoxidase) and (D) cnsK (acyltransferase). The role of the enzyme encoded by cnsC (P450) was also investigated and shown in (E). In each set, the UV (λ=270 nm) trace is shown above and the selected ion monitoring MS trace is shown below with the masses indicated in diffrent colors.
