Table 1.
Quantitation of NAD+ and NADH contents (amol/cell) via HPLC with UV detection (UV) and HPLC with 14C detection (AMS) for wild type, qpt1Δ and npt1Δ knockout yeast under both normal and CR growth conditions. The ratio of AMS to UV-quantitated values under the two growth conditions is also shown (AMS:UV). Each value is the mean and standard deviation of at least three replicate experiments (n = 3–6).
| Normal (2% Glucose) | Calorie-Restricted (0.5% Glucose) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| NAD+ | NADH | NAD+ | NADH | |||||||||
| UV | AMS | AMS:UV | UV | AMS | AMS:UV | UV | AMS | AMS:UV | UV | AMS | AMS:UV | |
| Wild Type | 82 (10) | 86 (12) | 1.1 (0.2) | 85 (8) | 88 (15) | 1.0 (0.1) | 54 (4) | 56 (5) | 1.0 (0.1) | 20 (7) | 21 (8) | 1.0 (0.1) |
| qpt1Δ knockout | 53 (10) | 58 (9) | 1.1 (0.1) | 9 (2) | 9 (1) | 1.1 (0.2) | 31 (4) | 35 (3) | 1.1 (0.1) | 15 (3) | 15 (3) | 1.0 (0.1) |
| npt1Δ knockout | 37 (5) | 0.1 (0.1) | 0.00 (0.01) | 5 (2) | 0.04 (0.02) | 0.01 (0.01) | 22 (2) | 0.00 (0.02) | 0.00 (0.01) | 3 (2) | 0.01 (0.03) | 0.00 (0.01) |