Table 2.

Previously reported total cellular NAD⁺ and NADH concentrations (mM) and ratios together with those from this study for wild type yeast, npt1Δ and qpt1Δ knockout strains under normal (2% glucose) and calorie-restricted (0.5% glucose) growth conditions.

	NAD+ (mM)		NADH (mM)		NAD+:NADH
	Normal	Calorie-Restricted	Normal	Calorie-Restricted	Normal	Calorie-Restricted
Present study Wildtype	1.2 (0.1)	0.8 (0.1) e)	1.2 (0.1)	0.3 (0.1) f)	1.0 (0.2)	2.7 (1.3) i)
Sporty et al. [2008]	1.4 (0.2)	1.7 (0.3)	1.4 (0.2)	0.9 (0.2)	1.0 (0.1)	1.9 (0.30)
Lin et al. [2004]	1.3 (0.1)	1.2 (0.1)	0.9 (0.1)	0.4 (0.1)	1.5	3.1
Anderson et al. [2002] a)	2	-	0.8	-	2.6	-
Present study qpt1Δ knockout	1.3 (0.3) c)	0.8 (0.1) c) e)	0.2 (0.1) b)	0.4 (0.1) c) g)	6.2 (2.4) b)	2.1 (0.7) c) i)
Present study npt1Δ knockout	0.9 (0.1) b)	0.5 (0.1) b) e)	0.13 (0.05) b)	0.08 (0.05) b) h)	8.1 (3.1) b)	6.6 (3.3) d) j)

^a)Reported values were converted to concentrations (mM) as previously described (11),

^b)P < 0.05 compared to present study wild type,

^c)P > 0.1 compared to present study wild type,

^d)0.05 < P< 0.10 compared to present study wild type,

^e)P < 0.05 compared to NAD⁺ under normal growth,

^f)P < 0.05 compared to NADH under normal growth,

^g)0.05 < P< 0.10 compared to qpt1Δ NADH levels under normal growth,

^h)P > 0.10 compared to npt1Δ NADH levels under normal growth,

ⁱ⁾P < 0.05 compared to NAD⁺:NADH ratio under normal growth conditions,

^j)P > 0.1 compared to NAD⁺:NADH ratio under normal growth.