Figure 6. Effects of the Kv7 channel inhibitor, linopirdine (Lino) on the cerebral artery SMC K⁺currents.

A. Representative traces of outward K⁺ currents in cerebral artery VSMCs evoked by steps (300 ms) to voltages over the range -70 to +70 mV with an initial holding potential of -70 mV. The Kv7 channel inhibitor, linopridine (10 μM) decreased K⁺ currents; an effect not reversed by sequential application of retigabine (20 μM). B. Group I-V curves showing linopirdine decreases K⁺ currents (n = 6 mice, 22 cells). Insert shows a limited voltage range highlighting inward current at negative potentials (for E_Rev values see Table 3). 2 way, repeated measures, ANOVA with Bonferroni multiple comparisons post hoc test; *P< 0.05 compared to control. C. Normalized conductance plotted as a function of voltage. Curve fitting was performed using a Boltzmann function; V_1/2 values are shown in Table 3. D. Linopirdine-sensitive K⁺ current calculated by subtraction of currents recorded in the absence and presence of linopirdine. V_1/2 values for panels C and D are shown in Table 3.