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ITC data of the wild-type and mutant medaka CR4/5-TRBD interactionsa
| TRBD | CR4/5 | Kd (μM) |
|---|---|---|
| WT | WT | 0.76 ± 0.21 |
| Y503A | WT | 10 ± 2.2 |
| WT | U182A | >1,000 |
| WT | U182C | >1,000 |
| WT | U182G | >1,000 |
| F376A | WT | >1,000 |
| WT | G189C | >1,000 |
| WT | G189U | >1,000 |
| WT | G189A | 175 ± 47 |
| WT | A199G | >1,000 |
| WT | A199U | >1,000 |
| WT | A199C | >1,000 |
Additional data in Supplementary Figure 3d. WT, wild type.
