Figure 6. 56Fe irradiation induces clonal expansion of club progenitor cells.
(A) Patches of GFP lineage tags (cytoplasmic RFP, nuclear GFP, cytoplasmic YFP, membrane CFP) assessed by immunofluorescence of GFP in formalin-fixed lung tissue from tamoxifen treated Scgb1a1-CreER™; Rosa26R-Confetti mice exposed to 2.5 Gy 600 MeV/nucleon 56Fe and recovered for 70 days. (B) Quantitation of lung epithelial patches (1–2 or 3+ cells that share the same lineage tag) from unirradiated and 56Fe irradiated mice. Arrows indicate tagged cells and asterisks indicate patches. Data is presented as the relative frequency (± SEM) of patches (1–2 vs 3+) per all lineage tagged airway epithelial cells. Significance to unirradiated controls indicated by: *** (p ≤ 0.001).