Table 7.
Effect of indacaterol on the potency and ability of fluticasone furoate and GW 870086X to promote gene expression in 2× GRE BEAS-2B reporter cells
| Gene | GILZ | CRISPLD2 | p57kip2 | PDK4 | ||||
|---|---|---|---|---|---|---|---|---|
| Treatment | p[A]50 (M) | Maximum induction (fold) | p[A]50 (M) | Maximum induction (fold) | p[A]50 (M) | Maximum induction (fold) | p[A]50 (M) | Maximum induction (fold) |
| Indacaterola | ND | 0.88 ± 0.1 | ND | 17.5 ± 1.1** | ND | 2.60 ± 0.3 | ND | 1.30 ± 0.2 |
| Fluticasone furoate | 9.12 ± 0.08 | 62.6 ± 7.9 | 9.12 ± 0.06 | 12.0 ± 1.1 | 9.01 ± 0.09 | 12.5 ± 0.7 | 8.81 ± 0.11 | 11.8 ± 1.4 |
| Fluticasone furoate +indacaterol | 9.16 ± 0.07 | 80.0 ± 5.5 | 9.15 ± 0.10 | 78.6 ± 3.8* | 9.20 ± 0.02 | 33.9 ± 1.3* | 9.10 ± 0.07 | 58.0 ± 6.5* |
| Indacaterola | ND | 0.77 ± 0.1 | ND | 16.4 ± 2.7 | ND | 2.60 ± 0.8 | ND | 1.30 ± 0.3 |
| GW 870086X | 8.44 ± 0.06 | 65.8 ± 8.2 | 8.28 ± 0.07 | 14.9 ± 2.8 | 8.41 ± 0.12 | 9.70 ± 2.5 | 8.29 ± 0.23 | 4.20 ± 0.8 |
| GW 870086X +indacaterol | 8.26 ± 0.12 | 62.7 ± 9.9 | 8.37 ± 0.20 | 79.5 ± 5.2* | 8.37 ± 0.10 | 25.9 ± 6.1* | 8.08 ± 0.17 | 12.2 ± 2.3* |
Data were calculated from the E/[A] curves shown in Figure 8 and represent the mean ± SEM of six independent determinations with the exception of GW 870086X on p57kip2 and PDK4 where n = 4 and 5 respectively.
P < 0.05, significant enhancement by indacaterol of FF- and/or GW-induced gene expression – Student's two-tailed, unpaired t-test.
P < 0.05, significant induction of gene expression by indacaterol alone relative to untreated cells – Student's two-tailed, unpaired t-test.
A fixed concentration of indacaterol (100 nM) was added concurrently with the glucocorticoid and gene expression measured at 6 h.
ND, not determined.