Figure 4.

Lymphatic vessel organization did not appear to be different between non-Sjögren’s syndrome (A) and Sjögren’s syndrome (B) human minor salivary glands. Five-µm frozen human minor salivary gland tissue sections were fixed as described in the Materials & Methods. Expression of PECAM-1 and LYVE-1 was detected using immunofluorescence microscopy, as follows: mouse-anti-PECAM-1 (red); goat-anti-LYVE-1 (green) and Hoechst nuclear stain (blue). Stained structures (green) correspond to salivary gland lymphatic vessels. White outlines differentiate acinar regions and yellow arrows indicate ductal structures. The x–y cross section images were obtained using a Leica DMI6000B inverted fluorescence microscope. Images are representative of n=6 experiments. Scale, 100 µm.