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. 2015 May;63(5):329–339. doi: 10.1369/0022155415575028

Figure 4.

Figure 4.

Expression of Chrna7 mRNA. (A) qRT-PCR analysis of Chrna7 mRNA expression in Chrna7 knock out (KO) mice as compared to wild type (Wt) mice (C57Bl/6). Data represent the mean values of three independent experiments ± SD. (B) Ethidium bromide-stained agarose gel electrophoresis shows the qRT-PCR products. Lane 1 depicts the DNA ladder, lanes 2–3 and lanes 5–6 show the mRNA expression of Chrna7 in Wt and Chrna7 KO skin biopsies, respectively. The house keeping gene HPRT was used as an internal control (lanes 4 and 7). PCR quality control sterile water (without DNA) served as a negative control (lane 8).