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Western blotting of whole skin biopsy lysates after 2D SDS-PAGE detected with the Chrna7 ab23832 antibody. The protein lysates were pooled from each of three wild type (Wt) mice. Approximately 40 µg of protein were resolved on 2D gels and analyzed by western blotting for ab23832 (size, ~50 kDa; pI, ~5.5).
