Table 1.

Antibodies Tested for Specificity in Wild Type and Corresponding Knock-out Mice Skin.

AChR subunit	Company	Catalogue Number	Dilutions Tested	Epitope
Rabbit anti-Chrm3	Abcam (Cambridge, UK)	ab41169	1:50; 1:100; 1:200; 1:400	C-terminal region
Goat anti-Chrm3	Santa Cruz Biotechnology (Dallas, TX)	sc-31486	1:50;1:100;1:200; 1:250; 1:400	N-terminal extracellular domain
Goat anti-Chrm3	Santa Cruz Biotechnology	sc-31487	1:50;1:100;1:200; 1:250; 1:400	Cytoplasmic domain
Rabbit anti-Chrm3	Sigma-Aldrich (Steinheim, Germany)	M0194	1:100; 1:200; 1:400	Amino acids 461–479 (3rd intra cellular loop)
Rabbit anti- Chrm3	Genetex (Irvine, CA)	GTX111637	1:100; 1:200	Amino acids 286–501
Rabbit anti-Chrna7	Abcam	ab28741	1:100; 1:200	Amino acids 398–447 (exon 10, within deleted region in Chrna7 KO mice)
Rabbit anti-Chrna7	Santa Cruz Biotechnology	sc-5544	1:100; 1:200; 1:400	Amino acids 367–502 (exon 10, within deleted region in Chrna7 KO mice)
Rabbit anti-Chrna7	R&D Abs (Las Vegas, NV)	AS-5631S	1:100; 1:200	Amino acids 493–502 (exon 10, within deleted region in Chrna7 KO mice)
Rabbit anti-Chrna7	Abcam	ab23832	1:100; 1:200	Amino acids 1–100 (exons 1–4, not in deleted region in Chrna7 KO mice)
Rabbit anti β-enolase	LSBio (Eching, Germany)	LS-C81180	1:200; 1:400; 1:800	N-terminal region
Rabbit anti β-actin	Abcam	a8227	1:100; 1:200; 1:400	Amino acids 1–100

AChR, acetylcholine receptor.