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List of Primers and Probes Used for qRT-PCR.
| Gene | Primer Sequence |
|---|---|
| Chrna7 (exons 1–4) | Forward: CCTGCAAGGCGAGTTCC |
| Reverse: CTCAGGGAGAAGTACACGGTGA | |
| Taqman probe: 6FAM-CTGGTCAAGAACTACAACCCGCTGGA–BBQ | |
| Chrna7 (exons 8–10) | Forward: GCCCTTGATAGCACAGTACTTCG |
| Reverse: GATCCTGGTCCACTTAGGCATTT | |
| Taqman probe: 6FAM-CAGTGGTCGTGACAGTGATTGTGCTGC-BBQ | |
| HPRT | Forward: GTTGGATACAGGCCAGACTTTGT |
| Reverse: CACAGGACTAGAACACCTGC | |
| Taqman probe: 6FAM-TTGCAGATTCAACTTGCGCTCATCTT–BBQ |
