Increased expressions of pro-inflammatory factors in SPC-A1 cocultured with M2-type macrophages. (A) mRNA expressions of IL-1β, IL-6 and IL-8 in SPC-A1 cocultured with M2-type macrophages for 24 and 48 h by RT-PCR. *, P<0.05 vs. SPC-A1 cultured alone for 24 and 48 h; (B) Supernate protein expressions of IL-1β, IL-6 and IL-8 in SPC-A1 cocultured with M2-type macrophages for 24 and 48 h by CBA assay. IL-1β, IL-6 and IL-8 expressions were significantly enhanced in SPC-A1 after being cocultured with M2-type macrophages for 24 and 48 h. *, P<0.05 vs. SPC-A1 cultured alone for 24 and 48 h. IL, interleukin.