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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Nat Genet. 2014 Oct 19;46(12):1343–1349. doi: 10.1038/ng.3119

Figure 2.

Figure 2

Overview of the CPT-Seq workflow. There are three key steps: (I) indexed transposition, (II) pooling, diluting and compartmentalization, and (III) indexed PCR. A set of 96 different indexed transposome complexes are used to set up 96 independent transposition reactions to create separate genomic virtual partitions (step I). Transposition reactions are pooled together, diluted to sub-haploid DNA content, and split to 96 compartments (step II). Upon removal of the transposase with SDS, compartment-specific libraries are generated using indexed PCR (step III). All samples are pooled together after PCR, and prepared for sequencing.