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. Author manuscript; available in PMC: 2015 Apr 26.
Published in final edited form as: J Comp Neurol. 2013 Oct 15;521(15):3570–3583. doi: 10.1002/cne.23372

Figure 3.

Figure 3

Pre-embedding immunogold labeling in stratum radiatum after delayed fixation. A: CaMKII labeling is found both in dendritic shafts and spines; labeling in spines seems stronger than after quick fixation. B: Characteristic electron-dense spheroids in the dendritic shaft (arrowheads) are decorated with CaMKII labeling. To determine whether CaMKII reorganizes rapidly with hypoxia, spines and their parent shafts from brains fixed after 5-minute perfusion with saline were analyzed as for quick-fix tissue. C: Unlike in the quick-fix tissue, labeling density in the delay-fix material was similar for shaft and spine heads (n.s., not significant). D: The total number of particles per spine head was significantly larger in delay-fix than quick-fix spines (*P < 0.05, t-test). Scale bar = 500 nm in B (applies to A,B).