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IgG isotyping of anti-OVA antibodies resulting from mouse vaccinations with MUC1–KLH (2.5 μg), and OVA (20μg) in the presence of various saponin adjuvants.a
| adjuvant | IgG1 | IgG2a | IgG2b | IgG3 |
|---|---|---|---|---|
| 1/2 (SQS-21) | +++ | +/− | ++ | − |
| 4 (SQS-0-0-5-18) | +++ | − | ++ | − |
| 5 (SQS-1-0-5-18) | ++ | − | +/− | − |
| 9 (SQS-0-0-5-19) | +++ | +/− | + | − |
| 10 (SQS-1-0-5-19) | +++ | − | ++ | − |
Isotype titer levels: − = 0; +/− = 1–80; + = 81–160; ++ = 161–1280; +++ = >1280
