Figure 1. Bortezomib specifically induces IL-8 mRNA expression in LPS-stimulated U937 macrophages.
U937 cells were differentiated with 10 ng/ml PMA for 24 hours, and stimulated with LPS (1 μg/ml) in the absence or presence of 100 nM BZ. mRNA levels were measured by quantitative RT-PCR using β-actin as a control. The values represent the mean +/− SE of four experiments. Asterisks denote a statistically significant (p<0.05) change compared to LPS alone.