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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Biochem Biophys Res Commun. 2015 Mar 16;460(2):375–379. doi: 10.1016/j.bbrc.2015.03.041

Figure 3. BZ increases p38 nuclear accumulation in LPS-stimulated U937 macrophages.

Figure 3

Western blotting of cytoplasmic (CE) and nuclear extracts (NE) prepared from U937 cells differentiated with 10 ng/ml PMA for 24 hours, and stimulated with LPS (1 μg/ml) in the absence or presence of 100 nM BZ. The western blots were analyzed by using IKKα, IKKβ, and p38 MAPK specific antibodies. The presence of cytoplasmic proteins in nuclear fraction was evaluated by re-probing the membrane with lactate dehydrogenase (LDH) antibody. Nuclear contamination in the cytoplasmic fraction was assessed by using lamin B specific antibody. To confirm equal protein loading, the membrane was stripped and re-probed with actin antibody.