Figure 3.

Superimposed images of flagellar movement of demembranated, reactivated bull spermatozoa attached to a glass slide by their heads. The demembranated spermatozoa were reactivated with 3.5 mM MgATP²⁻. Free-Ca²⁺ concentration was adjusted to be 10⁻⁵ M (A), 10⁻⁷ M (B), and 10⁻⁹ M (C and D). D: 1.0 mM cAMP was added to the reactivation solution containing 10⁻⁹ M Ca²⁺. Bar, 20 µm.