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Effect of Ca2+ on sliding displacement of doublet microtubules extruded from the sperm flagella. Free-Ca2+ concentration was adjusted to be 10−4 M (A), 10−7 M (B), and 10−9 M (C). At higher Ca2+ concentrations, the extruded microtubules slid entirely off the sperm flagella. Bar, 10 µm.
