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. Author manuscript; available in PMC: 2015 Oct 20.
Published in final edited form as: Nat Commun. 2015 Apr 20;6:6874. doi: 10.1038/ncomms7874

Fig. 5.

Fig. 5

Accumulation of radioactive sulfamethazine. E. coli BL21(DE3)ΔabgTΔpabA cells expressing YdaH show a significant decrease in [3H]-sulfamethazine accumulation when compared with cells carrying the empty vector. When transformed with plasmids expressing the mutant transporters, D180A, N390, W400A, P418A, R426A, D429A and N433A, the levels of intracellular [3H]-sulfamethazine accumulation were much higher than that of cells expressing wild-type YdaH. Error bars depict standard deviation for n = 3. “*” indicates values of BL21(DE3)ΔabgTΔpabA/pET15b and BL21(DE3)ΔabgTΔpabA cells expressing the mutant transporters that are significantly higher than that of BL21(DE3)ΔabgTΔpabA/pET15bΩydaH expressing wild-type YdaH (P < 0.001; student’s t-test).