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. Author manuscript; available in PMC: 2015 Oct 24.
Published in final edited form as: Nat Commun. 2015 Apr 24;6:6956. doi: 10.1038/ncomms7956

Figure 2. ET-BSPs and S1P upregulate genes that encode sphingolipids and chemokines.

Figure 2

Molecules expressed in MC38 cells (A and B). MC38 colon cancer cells were cultured with BSA, NT-BSPs or ET-BSPs (20 µg/ml) (A) or with vehicle/S1P (B) for 18 h. Real-time PCR analysis of gene expression (A; B, left panel) and ELISA analysis of PGE2 (right panel) in the supernatants. The data represent means ± SEMs. *p < 0.05, **p < 0.01 (Student’s t-test). (C) MC38 cells were cultured with conditioned medium from colonic macrophages treated with BSA, NT-BSPs or ET-BSPs, and the expression of the indicated genes was measured by qPCR. The data represent means ± SEMs (n = 5). *p < 0.05, **p < 0.01 (Student’s t-test).