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. Author manuscript; available in PMC: 2015 Oct 24.
Published in final edited form as: Nat Commun. 2015 Apr 24;6:6956. doi: 10.1038/ncomms7956

Figure 3. Bacteria-secreted particles mediate the induction of CCL20+PGE2+S1P+ exosomes.

Figure 3

(A–B) MC38 cells were cultured with conditioned medium (CM) from macrophages treated with BSA, NT-BSPs or ET-BSPs (20 µg/ml), and the levels of SPH (A) and CCL20 (B) in tumor exosomes were assessed by LC-MS/MS (A) or Western blot (B). The data represent means ± SEMs (n = 5). *p < 0.05, **p < 0.01 (Student’s t-test).

(C) Changes in the ratio of S1P and PGE2 in exosomes released from MC38 cells pre-treated with BSA, NT-BSPs or ET-BSPs in the absence or presence of SphK1 inhibitor (SK1-I, 5 µM) for 48 h. The data represent means ± SEMs. **p < 0.01 (Student’s t-test).

(D) Western blot analysis of CCL20 in exosomes derived from (C).