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. Author manuscript; available in PMC: 2015 Oct 24.
Published in final edited form as: Nat Commun. 2015 Apr 24;6:6956. doi: 10.1038/ncomms7956

Figure 8. MyD88 plays a role in the APC-IDEN–mediated induction of CCR6+CD4+Th17+ cells.

Figure 8

Figure 8

Figure 8

(A) Electron microscopy images of IDENs in the colons of wild-type B6 mice.

(B–C) Confocal images of 16S RNA and CD63. CD63 (red) and bacterial 16S RNA (green) inhabiting loose mucous layers in colon tissue (B) and APCmin/+ lymphoid follicles (C).

(D–E) Confocal images of APCmin/+ mouse CD63 in the epithelial lumen (D; top), the lumen of the crypts (D; bottom) and tumor tissue (E).

(F–G) WT and MyD88−/− mice were treated with CD3-specific antibodies. FACS analysis of PKH26+ cells in the peripheral blood after gavage with PKH26-labeled IDENs (F), confocal images of small intestine sections stained with mucin-2-specific antibody (G).

(H–I) Under Th17 cell culture conditions, naïve CD4+ T cells were cultured in the presence of BSA or IDENs. FACS analysis of the induced chemokine receptors (H) and ELISA of the production of cytokines (I).

(J) ELISA analysis of IDEN PGE2 (Left panel) and real-time PCR analysis of COX2 (Middle panel) from the small intestine, and a Western blot analysis of CCL20 in IDENs from mice treated with CD3-specific antibody (Right panel). The data (I and J) represent means ± SEMs (n=3). **p < 0.01 (Student’s t-test).