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. 2015 Apr 1;34(2):110–115. doi: 10.1089/mab.2014.0050

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Copyright 2015, Mary Ann Liebert, Inc.

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FIG. 2. — SDS-PAGE analysis of purified anti-CXCL4 monoclonal antibody. Eluted solutions from the protein G affinity column were estimated by Coomassie staining after 8% SDS-PAGE. (A) Non-reducing SDS-PAGE electrophoresis. Lane 2, flow-through fraction; lane 3, wash fraction; lanes 4–8, elution fractions. (B) Reducing SDS-PAGE electrophoresis. Lane 2, flow-through fraction; lane 3, wash fraction; lanes 4–8, elution fractions.