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Cloning and sequence analysis of 16D6-3 antibody. (A) Agarose gel electrophoresis of hybridoma 16D6-3 RNA. (B) Agarose gel electrophoresis of 16D6-3 variable region genes. Lane M, PCR marker; lanes 1–3, VL gene fragment; lane 4, negative control; lanes 5–7, VH gene fragment. (C, D) Amino acid and DNA sequences of 16D6-3 antibody. Rat VH (C) and VL (D) domains contained within the antibody are shown by overlining.
