FIG. 5.

Hybrid cords. (A) H&E staining of cord-containing tissue constructs resected after 7 days in vivo (boxes: location of individual cords, arrowheads: individual capillaries around periphery of cord). (B) H&E staining of cords made of hepatocytes only and hybrid Hep/EC cords resected after 7 days in vivo suggests the presence of blood in small vessels along the perimeter (dotted line) of the hybrid cords. (C) Sirius red/Fast green staining of collagen within cords suggests that the hepatocytes largely degrade the collagen core. (D) Ter-119 (erythrocytes, white), human-specific CD31 (HUVECS, red), and arginase 1 (Arg-1; hepatocytes, green) confirm the presence of blood encased in capillaries lined with human endothelium directly adjacent to viable hepatocytes in the hybrid cord condition (arrowheads: individual capillaries; scale bar, 50 μm). (E) Quantification of blood area, vessel diameter, and vessel numbers for hybrid cords. Error bars SEM, n≥20. Color images available online at www.liebertpub.com/tec