FIG. 1.

HIV Env binding profiles of MAbs. Human recombinant MAbs were added in triplicate to ELISA plates coated with 0.5 μg/mL of indicated HIV Env proteins and binding detected with anti-human IgG. (A) MAbs were tested at 10 and 1 μg/mL and relative binding indicated; shading indicates MAbs were isolated from the same subject. (B) MAbs were serially diluted and binding to indicated oligomeric gp140 determined, mean±SEM presented. MAbs b12, 17b, and 4E10 included as positive controls. Polyclonal human IgG included as negative control.