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HIV gp41 and V1V2V3 dependent binding of MAbs. Human recombinant MAbs were added in triplicate at 10 and 1 μg/mL to ELISA plates coated with (A) 2 μg/mL of YU2 gp41 or (B) 0.5 μg/mL of oligomeric YU2 gp140 ΔV1V2V3 and binding detected with anti-human IgG.