Table 2. The effects of PHE and sperm concentration on blastocyst developmental rate when using IVF medium including theophylline.
| Bulls | Sperm concentration (×106 cells/ml) |
PHE | No. of oocytes (Replicates) |
% cleavage | % blastocysts | Mean cell numbers in blastocysts (n) |
| A | 1 | + | 72 (3) | 81.9 ± 2.4 | 44.5 ± 8.7 | 144.9 ± 50.6 (33) |
| 2 | + | 71 (3) | 86.2 ± 8.8 | 50.8 ± 11.3 | 148.9 ± 62.3 (36) | |
| 5 | − | 70 (3) | 87.5 ± 12.5 | 54.6 ± 11.5 | 165.9 ± 64.4 (38) | |
| B | 1 | + | 112 (4) | 72.2 ± 6.3 | 36.6 ± 6.6 | 174.8 ± 60.3 (41) |
| 2 | + | 80 (3) | 78.3 ± 4.4 | 50.0 ± 13.3 | 183.9 ± 63.8 (40) | |
| 5 | − | 157 (6) | 81.5 ± 9.7 | 39.2 ± 10.0 | 169.1 ± 69.6 (24) | |
| C | 1 | + | 65 (3) | 68.0 ± 4.8 | 24.8 ± 15.2 | 148.3 ± 55.4 (16) |
| 2 | + | 91 (3) | 86.8 ± 3.1 | 31.9 ± 5.0 | 191.2 ± 77.0 (29) | |
| 5 | − | 189 (8) | 80.5 ± 11.5 | 30.4 ± 8.0 | 188.1 ± 76.1 (58) | |
Values are means ± SD. Embryos derived from bulls A to C were cultured in 40-μl droplets.