Table 1. Components of each medium used in this study.
| Components | PZM3 a) | PZM3+G | PZM3+F | PZM+G | PZM+F | PZM | |
| NaCl | mM | 108.00 | 105.22 | 105.22 | 105.42 | 105.42 | 108.20 |
| KCl | mM | 10.00 | 10.00 | 10.00 | 10.00 | 10.00 | 10.00 |
| KH2PO4 | mM | 0.35 | 0.35 | 0.35 | 0.35 | 0.35 | 0.35 |
| MgSO4•7H2O | mM | 0.40 | 0.40 | 0.40 | 0.40 | 0.40 | 0.40 |
| NaHCO3 | mM | 25.07 | 25.07 | 25.07 | 25.07 | 25.07 | 25.07 |
| Na-pyruvate | mM | 0.20 | 0.20 | 0.20 | – | – | – |
| Ca-lactate•5H2O | mM | 2.00 | 2.00 | 2.00 | – | – | – |
| Glucose | mM | – | 5.56 | – | 5.56 | – | – |
| Fructose | mM | – | – | 5.56 | – | 5.56 | – |
| CaCl2 | mM | – | – | – | 2.00 | 2.00 | 2.00 |
| L-glutamine | mM | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 |
| Hypotaurine | mM | 5.00 | 5.00 | 5.00 | 5.00 | 5.00 | 5.00 |
| EAA b) | ml/l | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| NEAA c) | ml/l | 10.00 | 10.00 | 10.00 | 10.00 | 10.00 | 10.00 |
| Kanamycin | mg/ml | 0.08 | 0.08 | 0.08 | 0.08 | 0.08 | 0.08 |
| BSA | mg/ml | 3.00 | 3.00 | 3.00 | 3.00 | 3.00 | 3.00 |
| Osmolality | mOs | 300.04 | 300.04 | 300.04 | 300.04 | 300.04 | 300.04 |
a) PZM3; Yoshioka et al., 2002 [11]. b) EAA; MEM essential amino acids solution (50 ×). c) NEAA; MEM nonessential amino acids solution (100 ×).