. 2015 Jan 19;61(2):116–122. doi: 10.1262/jrd.2014-130

Table 1. Outcome of heterospermic insemination with freshly ejaculated sperm and GSH-treated frozen-thawed sperm in Labrador retrievers.

Bitch no.	Days after LH surge				Profile of FT-TCI		Number of puppies
Bitch no.	3	4	5	6	Total volume of semen nseminated (ml) *	Post-thaw progressive motility (%)	FT-TCI	Fr-AI·NM
1	―	―	FT-TCI	NM	1.5	30	0	7
1	―	―	FT-TCI	FT-TCI	1.5	30	0	7
2	―	―	Fr-AI	Fr-AI	3	45	0	7
2	―	―	FT-TCI	FT-TCI	3	45	0	7
3	Fr-AI	Fr-AI	Fr-AI	Fr-AI	4.8	22.5	4	6
3	Fr-AI	FT-TCI	FT-TCI	Fr-AI	4.8	22.5	4	6
4	FT-TCI	FT-TCI	FT-TCI	FT-TCI	6	50	1	0

FT-TCI: transcervical insemination with frozen-thawed semen. Fr-AI: artificial insemination with fresh ejaculate. NM: natural mating. * Concentrations of GSH-treated frozen-thawed sperm: 100 × 10⁶ sperm/ml.