Fig. 1.

Construction of the retroviral vector for generation of transgenic mice. (A) The retrovirus vector was designed to express the four stemness factors by tetracycline-mediated induction. For further details, see the Methods section. (B) Confirmation of transgenic mice using four-factor PCR. Red dotted lines indicate transgenic mice. (C) Western blotting analysis of the four stemness factors with tetracycline-mediated induction in control and transgenic mouse-derived fibroblasts. Fibroblasts were isolated and cultured by adding doxycycline for 10 days and then subjected to Western blot analysis using each antibody.