Table 1.
Parameters for the HT:aptamer blockades to Type I ClyA-CS and Type II ClyA-CS nanopores. Ires% is the residual current, τoff the lifetime, koff,0 mV the extrapolated dissociation rate at 0 mV, ΔU is the reduction in the HT:TBA dissociation energy barrier due to the applied potential V and Kd the complex dissociation affinity constant. Errors are stated as standard deviations.
| Ires% | τoff (ms) | ΔU (kcal mol−1 V−1) | koff,0 mV (s−1) | Kd (nM) | |
|---|---|---|---|---|---|
| Type II ClyA-CS | |||||
| TBA (L1a) | 45.7±0.9 | 1019±179 | 15.5±3.7 | 2.57±2.10 ×10−2 | 13±3 |
| TBA (L1b) | 53.6±0.9 | 25±2 | 8.5±1.0 | 5.31±1.16 | |
| TBA-T3A (L1a) | 45.6±0.5 | 3301±1717 | 17.2±6.1 | 5.83±7.80 ×10−3 | 6±1 |
| TBA-T12A (L1b) | 53.8±0.3 | 52±5 | 11.0±1.9 | 1.46±0.62 | 7±1 |
| TBA-ΔT3 (L1a) | 46.0±1.3 | 348±57 | 14.2±1.9 | 9.26±3.89 ×10−2 | 22±4 |
| TBA-ΔT12 (L1b) | 54.5±1.1 | 9±1 | 6.9±0.2 | 21.51±1.07 | 54±29 |
| Type I ClyA-CS | |||||
| TBA (L1a) | 35.6±0.7 | 118±15 | 10.0±1.7 | 0.90±0.34 | 8±1 |
| TBA (L1b) | 62.0±0.8 | 386±18 | 2.5±0.2 | 1.40±0.07 |