Table 3. Statistics of germination of the ΔkexB mutant.
| Time (h) | Wild-type Number of germ tube |
ΔkexB Number of germ tube |
Complemented strain Number of germ tube |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
| ||||||||||||
| 0 | 1 | 2 | hyper-branching | 0 | 1 | 2 | hyper-branching | 0 | 1 | 2 | hyper-branching | |
| 5 | 85±3 | 15±1 | 0 | 0 | 72±2 | 27±1 | 1±0 | 0 | 88±5 | 12±1 | 0 | 0 |
| 6 | 45±1 | 54±2 | 1±0 | 0 | 41±2 | 57±4 | 2±0 | 0 | 45±3 | 54±5 | 1±0 | 0 |
| 7 | 7±1 | 78±4 | 15±1 | 0 | 14±1 | 72±3 | 13±1 | 1±0 | 6±1 | 80±6 | 14±2 | 0 |
| 8 | 4±0 | 73±5 | 22±3 | 1±0 | 6±0 | 60±2 | 14±1 | 20±2 | 1±0 | 67±3 | 30±2 | 2±0 |
Freshly harvested conidia (106) were poured into a Petri dish containing five glass coverslips and incubated in 10 ml of complete liquid medium at 37°C. The coverslips with adhering germlings were removed and counted under microscope. For each independent experiment 100 conidia were counted and three independent experiments were carried out.