Table 4. Cell wall components of the ΔkexB mutant at 37°C and 50°C.
| Temperature | Strain | Alkali soluble | Alkali insoluble | ||
|---|---|---|---|---|---|
|
|
|||||
| Glycoprotein (μg) | α-glycan (μg) | β-glycan (μg) | Chitin (μg) | ||
| 37°C | WT | 148±6 | 71±3 | 258±13 | 204±11 |
| M | 106±3 | 43±3 | 349±18 | 262±4 | |
| C | 153±12 | 58±1 | 294±4 | 221±9 | |
| 50°C | WT | 137±9 | 171±5 | 977±30 | 482±7 |
| M | 152±6 | 90±7 | 700±23 | 439±8 | |
| C | 128±10 | 123±4 | 771±14 | 452±11 | |
Three aliquots of 10 mg lyophilized mycelia were used as independent samples for cell wall analysis, and the experiment was repeated three times from different biological samples. The values shown are microgram of cell wall component per 10 mg dry mycelia (± SD). WT, wild-type; M, mutant; C, complemented strain.