. Author manuscript; available in PMC: 2016 Apr 21.

Published in final edited form as: Biochemistry. 2015 Apr 7;54(15):2530–2538. doi: 10.1021/acs.biochem.5b00135

Table 1.

IC₅₀ and K_i values for compounds 4, 7-13 against iNOS

Compound	IC₅₀(μM)^a	K_i(μM)^b
4 (L-NIO)	8.1	3.65
7	0.4	0.18
8	16.1	7.25
9	19.1	8.61
10	138	65.18
11	68.7	30.95
12	323	146
13	5333	2402

The apparent IC₅₀ values are represented as the mean from two or more independent experiments performed in duplicate with five or six data points each and correlation coefficients of 0.92-0.99. The experimental standard deviations were less than 10%.

The apparent K_i values were obtained by measuring the percent enzyme inhibition in the presence of 10 μM l-arginine with at least five concentrations of inhibitor. The parameters of the following inhibition equation⁴² were fitted to the initial velocity data: % inhibition = 100[I]/([I]+K_i(1+[S]/K_m)). K_m value for l-arginine was 8.2 μM (iNOS).