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. Author manuscript; available in PMC: 2015 Oct 24.
Published in final edited form as: Nat Commun. 2015 Apr 24;6:6910. doi: 10.1038/ncomms7910

Figure 3. Dnmt1 deletion suppresses mammary tumorigenesis.

Figure 3

Dnmt1Δ/Δ mice were crossed with MMTV-Neu-Tg and C3(1)-SV40-Tg mice to generate MMTV-Neu and C3(1)-SV40 transgenic mice in wild-type and Dnmt1-null genetic backgrounds. Using these mice, we monitored a, tumor incidence, b, age of the animals at tumor onset, c, tumor size, d, lung metastasis, and e, f, average survival time. Data are mean ± SEM (n= 24 mice in each group). g–h, Representative contour blots show reduced cancer stem cells (CSC, CD49f+CD24+) in Dnmt1Δ/Δ-MMTV-Neu and Dnmt1Δ/Δ-C3(1)-SV40 mice compared to their respective controls. Bar diagrams represent percent cancer stem cell populations of respective mouse mammary tumors. Data are mean ± SEM (n= 5 mice in each group). Statistical analysis was performed using unpaired Student's t-tests. Error bards represent SEM of independent experiments.