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. 2015 Mar 14;8:10. doi: 10.1186/s13072-015-0003-y

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© Marcho et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Mid-gastrulation expression and DNA methylation. (A) Four approximately E7.0 embryos from a single litter shown in age order - analyzed for Igf2r (B), Airn and ActB (C) expression as well as DMR2 DNA methylation (D). Igf2r is expressed from both alleles in all four embryos but Airn is detected only in the two older embryos (3 and 4). DNA methylation has already spread across DMR2 (D) independent of Airn expression. Filled circles = methylated cytosine, Open = unmethylated cytosine. (E) Schematic of Airn illustrating Igf2r start site and locations of primer pairs used in (F). (F) RT-PCR expression analysis from approximately E7.0 embryos 1, 3, 4, as well as other stages and adult kidney RNA highlight progressive expression of Airn coordinated with development. Pat, paternal; Mat, maternal; +, kidney cDNA.