Figure 3.

Af23 and Ad23 inhibited proliferation and induced apoptosis in H460 cells. (A) H460 cells were treated with Af23, Ad23 or NDGA at different concentration (0.74, 2.22, 6.67, 20, and 60 μM) for 72 h. The viability of H460 cells were detected by MTT assay, and the IC₅₀ values of compounds were fitted with GraphPad. (B) Effects of Af23 and Ad23 on caspase activation in H460 cells. H460 cells were harvested and lysated after incubated with Af23 (10 μM), Ad23 (10 μM), or NDGA (10 μM) for 12 h. The levels of cleaved caspase-3 and cleaved caspase-9 were determined by western blot analysis. (C) Morphological changes and hoechst staining were observed in H460 cells cultured with and without Af23, Ad23, or NDGA at indicated concentrations for 12 h (200×). The figures were representative of more than three separate experiments. D. Af23 and Ad23 induced cell apoptosis in H460 cells. H460 cells were treated with Af23, Ad23, or NDGA at indicated concentrations for 24 h, and then stained with Annexin V and PI, followed by detection using flow cytometry. The representative pictures are shown.