Figure 4.

Anti-tumor effects of compounds Af23 and Ad23 in H460 xenograft models. Xenografts were established in nude mice. Two days after the mice were treated with liposome vehicle (once daily, i.p.), Af23 (once daily, i.p., 5 mg/kg/day) or Ad23 (once daily, i.p., 5 mg/kg/day) for 28 days (n = 10 in each group). (A) Tumor volume (mm³) and (B) Tumor weight (g) were recorded (n = 10). Points, mean of 10 mice; bars, SD. **P < 0.01. (C) pFGFR1 expressions in tumor tissues were detected by Western Blot with FGFR1 as internal control (3 mice in each group were used); (D) Body weight of each group was recorded (n = 10 in each group); (E) The levels of p-ERK, p-AKT, Bcl-2, CyclinD1, and COX-2 in tumor tissues were detected by immunohistochemical staining (6 mice in each group were used). Representative pictures are shown.