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. 2015 May 1;18(5):542–548. doi: 10.1089/jmf.2014.3251

FIG. 4.

FIG. 4.

Effects of FRGE treated with β-glycosidase 1 (Bgp1) on ALP, BMP-2, Col-I, and on Runx-2 mRNA expression level in MC3T3-E1 cells were examined by reverse-transcriptase polymerase chain reaction. MC3T3-E1 cells were treated with (1, 10, and 100 μg/mL) or without FRGE fermented by β-glycosidase 1 (Bgp1) for 12 days. The GAPDH mRNA level was analyzed in the same samples as a reference gene. Each value is the mean±SEM of three to five independent experiments. **P<.01, ***P<.001 control versus RGE, ##P<.01, ###P<.001 RGE versus FRGE. Hence, 10 μg/mL provides a significant effect on ALP, BMP-2, Col-I, and on Runx-2 expression in MC3T3-E1 cells. BMP, bone morphogenetic protein; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.